本试剂盒采取直接竞争法:酶标板上包被的抗原与加入的校准品(或样本)中的抗原竞争结合加入的酶标抗体;洗去多余酶标抗体,用TMB底物显色;样本吸光值与其残留物孔雀石绿呈负相关,与校准曲线比较再乘以其对应的稀释倍数,即可得出样品中孔雀石绿的含量。
二、适用范围
可定性定量检测水产样本等样本中孔雀石绿的残留量。
三、交叉反应率
显性孔雀石绿················································100%
隐性孔雀石绿·················································0.1%
结晶紫 ·······················································95%
隐性结晶紫····················································0.1%
四、提供的材料与试剂
1、96孔酶标板×1块(包被有偶联抗原)
2、标准液×6瓶:(1ml/瓶)
0ppb,0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb
3、高浓度标准品:(1ml/瓶)50ppb
4、酶标抗体 7ml
5、底物A液 7ml
6、底物B液 7ml
8、终 止 液 7ml
9、20×浓缩洗涤液 50ml
10、氧化剂 10ml
五、检测方法灵敏度、准确度、精密度
试剂盒灵敏度:0.05ppb
样本最低检测限:
水产样品···························································0.15ppb
准确度:
水产样品······················································75%~120%
精密度:
试剂盒的变异系数均小于10%。
六、贮藏条件及保存期
贮藏条件:保存试剂盒于2~8℃。
保存期:该产品有效期为12个月。