来自甘肃省商业科技研究所流通领域动物源性制品质量安全技术创新服务平台和甘肃省动物源性制品安全分析与检测技术重点实验室的Yingwen Qian、Xia Hong和Caixia Yuan等人开发了用于测定猪肉中β2-兴奋剂的可靠且廉价的预处理程序。该方法使用由石墨烯（rGO）功能化的多壁碳纳米管（CNT）的纳米复合材料作为反向分散吸附剂。通过高效液相色谱纯化后的分析，优化了纳米复合材料的提取时间和性能。在优化条件下，本方法在浓度范围内具有线性响应。猪肉样品中为0.5～50 ng/mL，检出限接近0.1 ng/mL。当前方法的精度（变异系数）低于5％，而回收率超过98.3％。
与rGO和CNT相比，纳米复合材料表现出高吸附性、长期储存稳定性、令人满意的抗干扰活性和对β2-兴奋剂的高选择性。
文章摘要
ABSTRACT
Development of a reversed dispersive based graphene functionalized with multiwalled carbon nanotubes for detection of β2-agonists in pork by high-performance liquid chromatography
Ying-Wen Qiana,b, Xia Honga,b, Cai-Xia Yuana,b,?, Xin-Kui Zhoua,b, Hai-Ning Hea,b,
Dong-Shuai Wanga,b
a Quality and Safety Technological Innovation Service Platform on Circulation of Animal Origin, Gansu Institute of Business and Technology, Lanzhou, Gansu 730010, China
b Key Laboratory of Animal Products Safety Analysis and Detection Technology of Gansu Province, Lanzhou, Gansu 730010, China
Abstract
A reliable and inexpensive pretreatment procedure in the determination of β2-agonists in pork was developped. The procedure used a nanocomposite of multiwalled carbon nanotubes (CNTs) functionalized with graphene (rGO) as the reversed dispersive sorbent. It was analyzed after purification by high-performance liquid chromatography, the extraction time and the properties of the nanocomposite were optimized. Under optimized conditions, present method has linear response over concentration range of 0.5–50 ng/mL in pork samples with a satisfactory detection limit close to 0.1 ng/mL. The precisions of the current method (coefficient of variation) are lower than 5%, while recoveries are more than 98.3%. The nanocomposite exhibited high adsorptivity, long-term storage stability, satisfactory anti-interfering activity and high selectivity toward β2-agonists compared with those of rGO and CNTs.
