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FOOD RES INT:益生菌乳清乳饮料对人前列腺细胞系的抗增殖和凋亡作用

放大字体  缩小字体 发布日期:2020-06-24
核心提示:益生菌乳清乳饮料对人前列腺癌细胞系PC-3和DU-145细胞系的细胞毒性活性。
   研究要点:
 
  益生菌乳清乳饮料对人前列腺癌细胞系PC-3和DU-145细胞系的细胞毒性活性。
 
  益生菌乳清乳饮料在PC-3和DU-145细胞系中引起广泛的凋亡诱导。
 
  益生菌乳清乳饮料在预防癌症中的治疗潜力。
 
  巴西里约热内卢州联邦大学(UniRio)功能食品实验室的Lana S.Rosa、Mariana L.Santos和Anderson J.Teodoro*等人通过本研究旨在评估益生菌乳清饮料对人前列腺癌细胞系(PC-3和DU-145)的抗增殖和凋亡作用。
 
  制作了5 种不同的乳清饮料:传统乳清饮料(不添加益生菌,CTL);以及包含嗜酸乳杆菌La-05、嗜酸乳杆菌La-03、干酪乳杆菌-01和动物双歧杆菌Bb-12的乳清饮料。通过MTT法和细胞周期阻滞(cell cycle arrest)检测细胞活力,并通过流式细胞仪检测细胞凋亡。所有样品均表现出对两种细胞系的细胞毒性活性。干酪乳杆菌-01、Bb-12和La-05乳清饮料(50.0和100.0 μg/mL)的PC-3细胞百分比在G0/G1期和S期中降低,随后在G2/M期升高。乳清饮料的提取物在两个细胞系中引起了广泛凋亡诱导,与益生菌菌株无关。然而,添加了干酪乳杆菌-01的乳清饮料可能是对抗前列腺癌细胞的更好的候选药物。
 
  Abstract
 
  Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines
 
  Lana S. Rosaa, Mariana L. Santosa, Joel P. Abreua, Celso F. Balthazarb, Ramon S. Rochabc, Hugo L. A. Silvab, Erick A. Esmerinob, Maria Carmela K. H. Duarteb, Tatiana C. Pimentelc, M?nica Q. Freitasb, Márcia C. Silvad, Adriano G. Cruzb, Anderson J. Teodoroa
 
  aUniversidade Federal do Estado do Rio de Janeiro (UNIRIO), Laboratório de Alimentos Funcionais, Rio de Janeiro 22290-240, Brazil
 
  bUniversidade Federal Fluminense (UFF), Faculdade de Veterinária, Niterói 24230-340, Brazil
 
  cInstituto Federal do Paraná, Campus Paranavaí, 87703-536 Paraná, Brazil
 
  dInstituto Federal de Educa??o, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Departamento de Alimentos, Rio de Janeiro 20270-021, Brazil
 
  The present study aimed to evaluate the antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cancer cell lines (PC-3 and DU-145). Five different whey beverages were manufactured: conventional whey beverage (without addition of probiotic strain, CTL); and whey beverages containing Lactobacillus acidophilus La-05, Lactobacillus acidophilus La-03, Lactobacillus casei-01, and Bifidobacterium animalis Bb-12. Cell viability was determined by MTT assay and cell cycle arrest, and apoptosis by flow cytometry. All the samples presented cytotoxic activities against both cell lines. A decrease in the percentage of PC-3 cells in G0/G1 and S, followed by an increase in G2/M phase were observed with L. casei-01, Bb-12 and La-05 beverages (50.0 and 100.0 μg/mL). The extracts of the whey beverages caused extensive apoptosis induction in both cells’ lines, regardless of the probiotic strain. However, the whey beverage added with L. casei-01 might be a better candidate against prostate cancer cells.
 
 
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