通过免疫印记和酶联免疫吸附测定证实，交联TM的IgG/lgE结合电位降低。酶促交联改善了RBL-2H3和KU812细胞的胃肠消化率并诱导了较低水平的脱粒。此外，交联TM可减少过敏性症状，并降低血清IgG1、IgE、组胺、类胰蛋白酶和mMCP-1水平。在脾脏细胞中，CLC+和CTG+下调Th2相关细胞因子、上调IFN-γ和IL-10。这些发现表明，通过影响构象结构、增强消化率、减少细胞脱粒过程并积极调节Th1/Th2免疫平衡，CTG+在减轻原肌球蛋白的致敏性方面显示出比CLC+更多的潜力。
Abstract
Immunomodulatory Effect of Laccase/Caffeic Acid and Transglutaminase in Alleviating Shrimp Tropomyosin (Met e 1) Allergenicity
Ishfaq Ahmed, Hong Lin, Lili Xu, Shuang Li, Joana Costa, Isabel Mafra, Guanzhi Chen, Xiang Gao, and Zhenxing Li*
This work aimed to investigate the effect of enzymatic cross-linking on the allergenic potential of shrimp tropomyosin (TM), Met e 1. The cross-linked TM with laccase (CL), laccase/caffeic acid (CLC and CLC+), and transglutaminase (CTG and CTG+) formed macromolecules and altered the allergen conformation. The IgG/IgE-binding potentials of the cross-linked TM were reduced as confirmed by Western blotting and ELISA. Enzymatic cross-linking improved the gastrointestinal digestibility and induced a lower level of degranulation in RBL-2H3 and KU812 cells. Moreover, cross-linked TM decreased anaphylactic symptoms, as well as reduced the serum levels of IgG1, IgE, histamine, tryptase, and mMCP-1. In spleen cells, CLC+and CTG+ downregulated the Th2-related cytokines and upregulated IFN-γ and IL-10. These findings revealed that CTG+ has shown more potential than CLC+ in mitigating the allergenicity of TM by influencing the conformational structure, enhancing the digestibility, decreasing the cellular degranulation process, and positively modulating the Th1/Th2 immunobalance.
