Mussel tissue - Organics
This Standard Reference Material® (SRM®) is intended for use in evaluating analytical methods for the determination of
selected polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyl (PCB) congeners, chlorinated pesticides,
polybrominated diphenyl ether (PBDE) congeners, hexabromocyclododecane (HBCD) isomers, methylmercury, inorganic
mercury, and total mercury in marine bivalve mollusk tissue and similar matrices. All of the constituents for which certified
and reference values are provided are naturally present in the freeze-dried mussel tissue. A unit of SRM2974A consists
of one bottle containing approximately 5 g of freeze-dried mussel tissue.
Certified Concentration Values for Selected PAHs in SRM2974A
                                                                    Mass Fraction
                                                        g/kg (dry-mass basis)                                                                         Mass Fraction
                                                                                                                                                       g/kg (dry-mass basis)
Phenanthrene ................................................. 74.4 ± 4.7    Benzo[c]phenanthrene .................................... 23.2 ± 1.9
1-Methylphenanthrene .................................... 17.6 ± 1.6   Benz[a]anthracene .......................................... 31.1 ± 3.9
2-Methylphenanthrene .................................... 28.2 ± 2.6   Chrysene and Triphenylene .......................... 123.6 ± 2.9
3-Methylphenanthrene .................................... 24.1 ± 1.4   Benzo[b]fluoranthene ...................................... 41.5 ± 2.6
9-Methylphenanthrene .................................... 15.9 ± 1.3   Benzo[j]fluoranthene ....................................... 21.4 ± 1.1
4-H-Cyclopenta[def]phenanthrene .............. 13.15 ± 0.71   Benzo[k]fluoranthene .................................. 18.95 ± 0.54
Fluoranthene ..................................................... 287 ± 34   Benzo[e]pyrene ............................................... 58.9 ± 2.9
Pyrene .............................................................. 166 ± 21    Benzo[a]pyrene ............................................. 9.73 ± 0.43
1-Methylpyrene ........................................... 10.69 ± 0.83   Perylene ....................................................... 6.80 ± 0.34
4-Methylpyrene ........................................... 19.77 ± 0.89   Benzo[ghi]perylene ......................................... 23.7 ± 2.2
Benzo[ghi]fluoranthene ................................... 18.7 ± 1.7

Certified Concentration Values for Selected PCB Congeners in SRM2974A
                                                                                                Mass Fraction
                                                                                   g/kg (dry-mass basis)
PCB 8 ............... 2,4'-Dichlorobiphenyl.......................................2.01 ± 0.08
PCB 18 ............... 2,2',5-Trichlorobiphenyl ................................. 4.03 + 0.22
PCB 44 ............... 2,2'3,5'-Tetrachlorobiphenyl ........................ 16.24 + 0.71
PCB 49 ............... 2,2',4,5'-Tetrachlorobiphenyl ........................... 17.1 + 1.2
PCB 52 ............... 2,2',5,5'-Tetrachlorobiphenyl ....................... 22.42 + 0.92
PCB 66 ............... 2,3',4,4'-Tetrachlorobiphenyl ........................... 20.6 + 1.1
PCB 70 ............... 2,3',4',5-Tetrachlorobiphenyl ....................... 15.45 + 0.64
PCB 74 ............... 2,4,4',5-Tetrachlorobiphenyl .......................... 9.02 + 0.37
PCB 87 ............... 2,2',3,4,5'-Pentachlorobiphenyl ................... 14.36 + 0.56
PCB 95 ............... 2,2',3,5',6-Pentachlorobiphenyl ................... 23.72 + 0.49
PCB 99 ............... 2,2',4,4'5-Pentachlorobiphenyl .................... 24.51 + 0.54
PCB 101 ............... 2,2',4,5,5'-Pentachlorobiphenyl ................... 39.84 + 0.96
PCB 105 ............... 2,3,3',4,4'-Pentachlorobiphenyl ................... 16.47 + 0.43
PCB 110 ............... 2,3,3',4',6-Pentachlorobiphenyl ................... 35.88 + 0.87
PCB 118 ............... 2,3',4,4',5-Pentachlorobiphenyl ....................... 42.9 + 2.1
PCB 128 ............... 2,2',3,3',4,4'-Hexachlorobiphenyl .................. 8.24 + 0.33
PCB 138 ............... 2,2',3,4,4',5'-Hexachlorobiphenyl .................... 61.5 + 2.3
PCB 146 ............... 2,2',3,4',5,5'-Hexachlorobiphenyl .................. 8.07 + 0.40
PCB 149 ............... 2,2',3,4',5',6-Hexachlorobiphenyl ................ 31.77 + 0.95
PCB 151 ............... 2,2',3,5,5',6-Hexachlorobiphenyl ................... 5.99 + 0.20
PCB 153 ............... 2,2',4,4',5,5'-Hexachlorobiphenyl .................... 78.8 + 2.5
PCB 156 ............... 2,3,3',4,4',5-Hexachlorobiphenyl ................... 5.80 + 0.25
PCB 170 ............... 2,2',3,3',4,4',5-Heptachlorobiphenyl .............. 2.04 + 0.08
PCB 177 ............... 2,2'3,3',4',5,6-Heptachlorobiphenyl ............... 5.48 + 0.20
PCB 180 ............... 2,2',3,4,4',5,5'-Heptachlorobiphenyl .............. 5.31 + 0.16
193 ............... 2,3,3',4',5,5',6-Heptachlorobiphenyl
PCB 183 ............... 2,2',3,4,4'5',6-Heptachlorobiphenyl ............... 7.06 + 0.26
PCB 187 ............... 2,2',3,4',5,5'6-Heptachlorobiphenyl ............. 15.52 + 0.48
PCB 194 ............... 2,2',3,3',4,4',5,5'-Octachlorobiphenyl ........ 0.485 + 0.040

Certified Concentration Values for Selected Chlorinated Pesticides in SRM2974A
                                                                Mass Fraction
                                                    g/kg (dry-mass basis)                                                                      Mass Fraction
                                                                                                                                                 g/kg (dry-mass basis)
Hexachlorobenzene ................................. 0.113 + 0.007      cis-Chlordane .............................................. 8.54 + 0.17
4,4'-DDE .................................................... 17.37 + 0.82       trans-Chlordane ........................................... 7.12 + 0.15
4,4'-DDD .................................................... 13.56 + 0.58      cis-Nonachlor ............................................... 1.91 + 0.10
4,4'-DDT ...................................................... 6.78 + 0.32       trans-Nonachlor ........................................... 5.60 + 0.39

Certified Concentration Values for Selected PBDE Congeners in SRM2974A
                                                                                                  Mass Fraction
                                                                                      g/kg (dry-mass basis)
PBDE 28 ........... 2,4,4'-Tribromodiphenyl ether ....................... 0.905 + 0.051
          33 ........... 2',3,4-Tribromodiphenyl ether
PBDE 49 ........... 2,2',4,5'-Tetrabromodiphenyl ether ................... 1.36 + 0.06
PBDE 99 ........... 2,2',4,4',5-Pentabromodiphenyl ether ............... 4.78 + 0.24
PBDE 153 ........... 2,2',4,4',5,5'-Hexabromodiphenyl ether ......... 0.201 + 0.014
PBDE 209 ........... Decabromodiphenyl ether ................................. 1.99 + 0.11

Certified Concentration Value of Methylmercury, Inorganic Mercury, and Total Mercury in SRM2974A
                                                                   Mass Fraction
                                                      g/kg (dry-mass basis)                                                                         Mass Fraction
                                                                                                                                                       g/kg (dry-mass basis)
Methylmercury ........................................... 69.06 + 0.81      Total Mercury ..................................................... 195 + 3
Inorganic Mercury .............................................. 122 + 3
Reference values for selected PAHs, PCBs and PBDE Congeners.