测定方法 比色法(570nm)或荧光法(530nm/590nm)定量测定过氧化物酶活性。
产品说明 过氧化物酶 (酶分类号 1.11.1.x)催化如下氧化还原反应:ROOR + 电子供体(2 e-) + 2H+ → ROH + ROH 许多过氧化物酶的最佳底物为过氧化氢(H2O2),而其他的则是机体内的过氧化物,如脂类过氧化物。 在细胞中,过氧化物酶能破坏有氧呼吸过程中形成的副产物毒性羟基。它作为一大族酶存在于动物、植物、真菌、细菌中。简单、直接、自动化的过氧化物酶测试方法受到了广泛的应用。本公司的过氧化物酶测试方法是利用过氧化氢与电子供体反应形成粉红色产物,其吸光强度(570nm)或荧光强度 (lexc = 530nm, lem = 590nm)可直接用于测定酶活性。
产品特点 检测范围:吸光法4 - 1000 U/L,荧光法0.8 - 25 U/L。
储藏条件:室温运输,缓冲液和显色剂在-20°C下保存,其余试剂在4°C下保存。
Description Peroxidases (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:ROOR + electron donor (2 e-) + 2H+ → ROH + ROH For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase).Simple, direct and automation-ready procedures for determining peroxidase activity find wide applications. our peroxidase assay uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (lexc = 530nm, lem = 590nm) is a direct measure of the enzyme activity.
Key features Use as little as 10 mL samples. Linear detection range: colorimetric assays 4 to 1000 IU/L, fluorimetric assays 0.8 to 25 IU/L peroxidase.
Storage conditions. The kit is shipped on blue ice. Store Assay Buffer and Dye Reagent at -20°C, all other reagents at 4°C. Shelf life of three months after receipt.
其他 :编号:DPOD-048,DPOD-100
测试:48,100T