然而，这些水解产物所发挥的有益效果并不都是一样的；因此，我们需要进一步的研究来确定哪些因素可以提高这些制剂的治疗适用性。来自加拿大阿尔伯塔大学农业、食品与营养科学系及心血管研究中心的Marina Offengenden、Subhadeep Chakrabarti和Jianping Wu使用不同的酶条件生成了许多具有不同肽谱的胶原蛋白水解产物。同时发现使用两种而非一种酶进行水解可以产生更多丰富的低分子量肽，进而改善生物活性。通过在人皮肤成纤维细胞上测试这些水解产物发现其对炎症变化、氧化应激、I型胶原的合成以及细胞增殖表现出了不同的作用。研究结果表明，不同的酶条件会影响水解产物的肽谱，并差异调节其生物活性和对真皮成纤维细胞的潜在保护作用。
结 论
研究结果表明，不同的酶处理产生的胶原蛋白水解产物的生物活性显着不同。 主要发现如下：
（a）不同的酶处理产生的肽的分子量和肽谱不同;
（b）双酶处理导致大量低分子量肽的产生;
（c）双酶产生的水解产物更有可能表现出抗炎和抗氧化活性；
（d）即使在双酶处理的制剂中，也仅有一些制剂可以诱导HDF的增殖，这表明它依赖于特定肽的特定作用而不仅仅是肽的大小或分子量。
文章摘要
ABSTRACT
Chicken collagen hydrolysates differentially mediate anti-inflammatory activity and type I collagen synthesis on human dermal fibroblasts
Marina Offengenden1, Subhadeep Chakrabarti1, Jianping Wu?
Department of Agricultural, Food & Nutritional Science and the Cardiovascular Research Centre, University of Alberta, Edmonton, AB, Canada
Abstract
Collagenis a major extracellular matrix protein. Given the potential anti-inflammatory and antioxidant profiles of these bioactive compounds, there has been increasing interest in using collagen derived peptides and peptide-rich collagen hydrolysates for skin health, due to their immunomodulatory, antioxidant and proliferative effects on dermal fibroblasts. However, all hydrolysates are not equally effective in exerting the beneficial effects; hence, further research is needed to determine the factors that improve the therapeutic applicability of such preparations. We used different enzymatic conditions togenerate a number of different collagen hydrolysates with distinct peptide profiles. We found that the use of two rather than one enzyme for hydrolysis generates a greater abundance of low molecular weight peptides with consequent improvement in bioactive properties. Testing these hydrolysates on human dermal fibroblasts showed distinct actions on inflammatory changes, oxidative stress, type I collagen synthesis and cellular proliferation. Our findings suggest that different enzymatic conditions affect the peptide profile of hydrolysates and differentially regulate their biological activities and potential protective response on dermal fibroblasts.
In this study, we used different enzymatic conditions to generate a range of collagen hydrolysates, characterize their constituent peptides and finally examine the corresponding biological actions on dermal fibroblasts. Our results suggest that different enzymatic treatments can generate collagen hydrolysates that markedly differ in their biological activities. The key findings were:
(a) different enzyme treatments generated peptides that differed in molecular weight and peptide profiles;
(b) dual enzyme treatments resulted in abundance of lower molecular weight peptides;
(c) dual enzyme generated hydrolysates were more likely to demonstrate anti-inflammatory and antioxidant activities；
(d) even among dual enzyme treated preparations, only some but not others could induce HDF proliferation, suggesting it was dependent on specific actions ofparticular peptide/s rather than peptide size/molecular weight alone.
